Effect of cadmium on morphometric traits, antioxidant enzyme activity and phytochelatin synthase gene expression (SoPCS) of Saccharum officinarum var. cp48-103 in vitro

Cadmium is an important environmental pollutant which genetically, physiologically and biochemically affects the cell. Phytochelatins (PC) are involved in one of the phytoremediation mechanisms, in which they are able to bind heavy metals, such as cadmium. The objective of this study was to evaluate morphometric, antioxidant enzyme activity, and SoPCS gene expression in sugarcane growing under cadmium stress. After propagation, samples were cultured in triplicate for 14 days in modified MS medium containing CdCl2 (100, 250, 500 µmol). The morphometric traits, pigments, quantity and antioxidant enzyme activity were studied in treated plantlets. SoPCS gene expression was analyzed by qRT-PCR. Growth traits decreased following cadmium treatment. The amount of Chla, Chlb and ChlT decreased in treated samples, whereas carotenoids increased significantly. A rapid increase in antioxidant enzyme activity was detected. Enhanced SoPCS gene expression was observed in treated roots, whereas gene expression pattern in leaves was irregular. In conclusion, cadmium decreases the photosynthetic mechanism and growth rate in sugarcane. Antioxidative enzymes and SoPCS gene expression were significantly unregulated in sugarcane roots compared to the leaves. Cadmium concentration in shoots and roots of sugarcane significantly increased. The management of cadmium bioaccumulation in non-edible tissues of sugarcane such as leaves and roots that are sometimes burned after harvest can be applied for environmental protection.