Additional file 1 of LncRNA AP000654.1 attenuates cerebral ischemia-reperfusion injury by regulating mitophagy-mediated NLRP3 inflammasome activation

Supplementary Material 1: Figure S1. Time-Dependent Induction of NLRP3 mRNA During OGD in HMC3 Microglia. Quantitative RT-PCR analysis of NLRP3 mRNA levels in HMC3 cells exposed to OGD for the indicated durations (0, 2, 4, 6, and 8 h) (n = 3). **P < 0.01, ***P < 0.001. Figure S2. Neuronal Localization and OGD-Responsive Downregulation of LncRNA AP000654.1. (A) RNA-FISH detection of lncRNA AP000654.1 (green) in neurons of mouse brain sections. Neurons were labeled with NeuN (red) and nuclei with DAPI (blue). NC, negative control AAV; OE, lncRNA AP000654.1-overexpressing AAV. scale bar, 20 µm. (B) Quantitative RT-PCR analysis of lncRNA AP000654.1 expression in HMC3 cells exposed to OGD for the indicated durations. Expression was significantly reduced during OGD (n = 3). *P < 0.05, **P < 0.01. Figure S3. Knockdown of LncRNA AP000654.1 Promotes OGD/R-Induced NLRP3 Inflammasome Activation, Pyroptosis Marker Expression, and Autophagy-Related Changes. Western blot analysis and quantification of NLRP3, p62, Beclin1, GSDMD-N, Caspase‑1 p20, and LC3B protein expression in HMC3 cells following OGD/R treatment and siRNA‑mediated knockdown of lncRNA AP000654.1 (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.