Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications

脂肪组织 体外 化学 脂滴 食品科学 脂肪细胞 生物化学 细胞生物学 生物
作者
John S.K. Yuen,Michael K. Saad,Ning Xiang,Brigid M Barrick,Hailey DiCindio,Chunmei Li,Sabrina W Zhang,Miriam L. Rittenberg,Emily T Lew,Kevin Lin Zhang,Glenn Leung,Jaymie A Pietropinto,David L. Kaplan
出处
期刊:eLife [eLife Sciences Publications Ltd]
卷期号:12 被引量:28
标识
DOI:10.7554/elife.82120
摘要

We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production.
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