Multilayer adsorption of lead (Pb) and fulvic acid by Chlorella pyrenoidosa: Mechanism and impact of environmental factors

When the multilayer adsorption of lead (Pb) and fulvic acid (FA) occurs on algal surface, the adsorption capacity of Pb on the algae will increase dramatically, thus increasing the environmental risk of Pb. However, the corresponding mechanism and the influence of environmental factors on the multilayer adsorption remain unclear. Here, microscopic observation methods and batch adsorption experiments were exactly designed to investigate the adsorption behavior of multilayer adsorption of Pb and FA on algal surface. The results of Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) revealed that carboxyl groups were the major functional groups responsible for the binding of Pb ions in multilayer adsorption, and its number was more than that in monolayer adsorption. The solution pH, with an optimal pH of 7, was a critical factor influencing the occurrence of multilayer adsorption because it influences the protonation of the involved functional groups and determines the concentration of Pb2+ and Pb-FA in the solution. Increasing the temperature was beneficial for multilayer adsorption, with ΔH for Pb and FA varied from +17.12 to +47.68 kJ/mol and +16.19 to +57.74 kJ/mol, respectively. The multilayer adsorption of Pb and FA onto algal surface also followed the pseudo-second order kinetic model, but was extremely slower than the monolayer adsorption of Pb and FA by 30 times and 15 orders of magnitude, respectively. Therefore, the adsorption of Pb and FA in the ternary system had a different adsorption behavior than that in the binary system, which verified the presence of multilayer adsorption of Pb and FA and further support the multilayer adsorption mechanism. This work is important to provide data support for water ecological risk prevention and control of heavy metals.