Bioefficacy of fungal chitin oligomers in the control of postharvest decay in tomato fruit

Tomato is one of the most commercialised and consumed fruits worldwide. However, tomatoes are highly susceptible to Alternaria rot. Among the safe strategies proposed to control Alternaria rot is the induction of defence mechanisms through biological elicitors, such as chitin. Chitin and its oligosaccharides are an activate plant defence mechanisms, but studies of fruits exposed to fungal chitin fragments are scarce. Therefore, the present work aimed to obtain and partially characterise chitin oligomers of Alternaria alternata, and evaluate their effect on the defence mechanism of tomato fruits and their tolerance to Alternaria rot. The chitin oligomers obtained had a molecular weight of ≤ 1 kDa, 12% N-acetyl-glucosamine, 0.2% residual protein, and were 94% acetylated. These oligomers markedly increased the enzymatic activity of chitinase and β-1,3-glucanase in tomato fruits, and the development of Alternaria rot was inhibited by 78%. Chitin oligomers of A. alternata represent a promising alternative to attenuate Alternaria rot in tomato fruits through an enzymatic defence mechanism.