苯丙素
MYB公司
类黄酮生物合成
生物
转基因水稻
苏贝林
转基因作物
单甘醇
生物化学
转基因
苯丙氨酸解氨酶
转录因子
生物合成
木质素
植物
基因表达
基因
转录组
苯丙氨酸
氨基酸
作者
Yue Yu,Dong‐Dong Guo,Donghong Min,Tao Cao,Ning Lei,Qiyan Jiang,Xianjun Sun,Hui Zhang,Wensi Tang,Shiqing Gao,Yongbin Zhou,Zhao-Shi Xu,Jun Chen,You-Zhi Ma,Ming Chen,Xiaohong Zhang
标识
DOI:10.1016/j.plaphy.2022.11.032
摘要
R2R3-MYB transcription factors play an important role in the synthesis of phenylpropanoid-derived compounds, which in turn provide salt tolerance in plant. In this study, we found that the expression of foxtail millet R2R3-MYB factor SiMYB16 can be induced by salt and drought. SiMYB16 is localized in the nucleus and acts as a transcriptional activator. Phylogenetic analysis indicates that SiMYB16 belongs to the R2R3-MYB transcription factor family subgroup 24. Transgenic rice expressing SiMYB16 (OX16) had a higher survival rate, lower malondialdehyde content, and heavier fresh weight compared with type (WT) under salt stress conditions. The transgenic plants also had a higher germination rate in salt treatment conditions and higher yield in the field compared with wild-type plants. Transcriptome analysis revealed that the up-regulated differential expression genes in the transgenic rice were mainly involved in phenylpropanoid biosynthesis, fatty acid elongation, phenylalanine metabolism, and flavonoid biosynthesis pathways. Quantitative real-time PCR analysis also showed that the genes encoding the major enzymes in the lignin and suberin biosynthesis pathways had higher expression level in SiMYB16 transgenic plants. Correspondingly, the content of flavonoid and lignin, and the activity of fatty acid synthase increased in SiMYB16 transgenic rice compared with wild-type plants under salt stress treatment. These results indicate that SiMYB16 gene can enhance plant salt tolerance by regulating the biosynthesis of lignin and suberin.
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