Response of soil microbial properties in the life cycle of potatoes to organic substitution regimes in North China

Organic substitution regimes (OSR) can improve soil microbial properties in crop production. However, the dynamic responses of soil microbial flora and its driving factors in crop growth periods under OSR are still unclear. Here, a 2-year fertilizer field trial was conducted to explore the response of soil chemistry and microbial properties to OSR in the life cycle of potatoes (Solanum tuberosum L.) in North China. The fertilization treatment included only mineral fertilizer (ConN), substitution of 30% mineral N with organic N (M30), and substitution of 60% mineral N with organic N (M60); soil samples were collected and analyzed in the early flowering period (EP), flowering period (MP), and maturity period (LP) of potatoes. As the potato growth period progressed, M30 and M60 increased soil organic carbon (SOC) compared with ConN. Soil total nitrogen (pH) increased (decreased) under M60, while soil available potassium was similar across three treatments. Compared with ConN, soil bacterial Chao, Shannon, and PD indexes significantly increased under M30 and M60 in MP and LP, and the fungal Shannon index increased in LP. Soil bacterial and fungal community structures under M30 and M60 were only significantly separated from ConN in MP. The bacterial communities of dominant phyla or genera responded more sensitively to the fertilization regimes than the flora of fungi, and the changes in the flora mainly occur in EP and MP. Redundancy analysis revealed that SOC and available phosphorus were highly correlated with soil microbial community compositions. Compared with ConN, soil microbial network complexity and fungal network robustness were higher under M30 and M60, while bacterial network robustness showed the opposite trend. Partial least squares pathway modeling showed that the conversion in the fertilization system or growth period had a direct positive effect on the C/N-cycling function of soil bacteria, but not on the C-degradation function of fungi. Our research has important theoretical implications for the development of microbial fertilizers and fertilization management in different potato growth periods.