生物传感器
化学
体内
离体
背景(考古学)
生物医学工程
再现性
线性范围
甘氨酸
间质液
生物物理学
检出限
体外
色谱法
生物化学
病理
医学
生物技术
古生物学
生物
氨基酸
作者
Qianyu Wang,Águeda Molinero-Fernández,Ana Casanova,Joep Titulaer,Jonatan C. Campillo-Brocal,Åsa Konradsson-Geuken,Gastón A. Crespo,María Cuartero
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-08-18
卷期号:94 (34): 11856-11864
被引量:11
标识
DOI:10.1021/acs.analchem.2c02317
摘要
Glycine (GLY) is gaining importance in medical diagnoses due to its relationship with multiple physiological functions. Today, GLY is exclusively analyzed using instrumentation centralized in clinical labs, and a tangible point-of-care tool that gathers real-time data from the patient for effective and fast evaluations is lacking. Relevant clinical advances are expected as soon as the rapid provision of both punctual and continuous measurements is possible. In that context, this work presents a microneedle (MN)-based biosensor for intradermal GLY detection in interstitial fluid (ISF). The MN tip is externally tailored to detect GLY levels through the hydrogen peroxide formed in its reaction with a quinoprotein-based GLY oxidase enzyme. The analytical performance of the MN biosensor indicates a fast response time (<7 s); acceptable reversibility, reproducibility, and stability; as well as a wide linear range of response (25–600 μM) that covers the physiological levels of GLY in ISF. The MN biosensor conveniently exhibits high selectivity for GLY over other compounds commonly found in ISF, and the response is not influenced by temperature, pH, or skin insertions. Validated intradermal measurements of GLY were obtained at the in vitro (with pieces of rat skin), ex vivo (on-body tests of euthanized rats) and in vivo (on-body tests of anesthetized rats) levels, demonstrating its ability to produce accurate physiological data. The developed GLY MN biosensor is skin-wearable and provides reliable, real-time intradermal GLY measurements in ISF by means of a minimally invasive approach.
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