细胞凋亡
化学
免疫印迹
促炎细胞因子
流式细胞术
芍药苷
龙胆属
传统医学
药理学
炎症
生物化学
分子生物学
生物
医学
色谱法
高效液相色谱法
基因
免疫学
作者
Peng Huang,Kai-Wen Kang,Deng-Yuan Huang,Chunlin Zhao,Hao Zheng,Yong-Xin Luo,Wen Yan,Ming-Feng Zou,Dongli Li,Rihui Wu,Yun-Cai Tian,Yuan Tian,Wenhuan Zhang,Jingwei Jin,Sheng Yin,Li‐She Gan
标识
DOI:10.1016/j.phytochem.2023.113920
摘要
Ten lignans, including six previously undescribed phenolic ester glycosyl lignans (1–6), were isolated from a well-known traditional Chinese medicine, Qin-Jiao, which is the dry root of Gentiana macrophylla Pall. (Gentianaceae). Their structures were determined by spectroscopic and chemical methods, especially 2D NMR techniques. Quantum chemical calculations of theoretical ECD spectra allowed the determination of their absolute configurations. Refer to its traditional applications for the treatment of rheumatic arthralgia and hepatopathy, these compounds were evaluated on a TNF-α induced MH7A human synoviocyte inflammation model and a D-GalN induced AML12 hepatocyte injury model. Compounds 1, 2, 5, and 6 significantly reduced the release of proinflammatory cytokine IL-1β in MH7A cells at 15 μM and they also could strongly protect AML12 cells against D-GalN injury at 30 μM. Flow cytometry and Western blot analysis showed that compound 5 ameliorated D-GalN induced AML12 cell apoptosis by upregulating the expression of anti-apoptotic Bcl-2 protein and down-regulating the expression of pro-apoptotic Bax protein.
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