Application of compost assisted by Fe3O4 nanoparticles in di (2-ethylhexyl) phthalate-contaminated soil remediation: Biostimulation strategy, Soil responses, and RSM/CCD Optimization

Globally, contamination of agricultural soils by phthalate esters (PAEs) caused by direct consumption of plastic mulch films has been confirmed. The most widely used plasticizer is di (2-ethylhexyl) phthalate (DEHP), which is a more recalcitrant endocrine-disrupting chemical (EDC). Because of its low solubility and hydrophobicity, it remains in the soil longer, causes bioaccumulation in agricultural products, and has negative repercussions for food safety. In this study, the performance of kitchen organic waste compost assisted by Fe3O4 nanoparticles in DEHP removal efficiency (%) and soil C:N ratio (two responses) was optimized using Response Surface Methodology (RSM) based on Central Composite Design (CCD) in Design-Expert software (11.0.3.0). Under optimum conditions, a DEHP concentration of 10 mg·kg-1 (dw soil), a retention time of 35 days, an NPs dose of 0.99 g·kg-1 (media), a removal efficiency of 91.6 %, and a soil C:N ratio of 10.5 with a desirability of 0.963 were determined. A quadratic model (P-value <0.0001, adjusted R2 = 0.974 (Y1), 0.943 (Y2)) was used to predict the variables and their interactions. The agricultural soil responses in the treatments amended by compost and Fe3O4 NPs (SCN) showed a significant increase in SOM, TC, TN, AP, K, and Fe nutrients when compared to the control (P < 0.05). After 35 days, in the SC1N3 treatment (DEHP concentration = 10 mg·kg-1, NPs dose =1.2 g·kg-1), with higher DEHP removal efficiency (89.57 %), the C:N:P ratio was equal to 100: 9.75:0.69, and the total microbial colony count was 3.6 × 109 CFU/ml at pH 7.45. The study found that compost nutrients and Fe-based nanoparticle micronutrients can enhance DEHP degradation by stimulating the soil's native microflora. As a result, the synergistic potential of compost and Fe3O4 nanoparticles can be considered a promising, cost-effective, and agri-environmentally friendly approach in the "assisted bioremediation" strategy of DEHP-contaminated soils.