基因组编辑
Cas9
清脆的
核糖核蛋白
计算生物学
生物
遗传学
基因
核糖核酸
作者
Yue Sun,Xiaojie Xu,Liwei Chen,Wei Leong Chew,Ping Yuan,Ali Miserez
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-08-16
卷期号:17 (17): 16597-16606
被引量:4
标识
DOI:10.1021/acsnano.3c02669
摘要
CRISPR/Cas9-based genome editing tools have enormous potential for the development of various therapeutic treatments due to their reliability and broad applicability. A central requirement of CRISPR/Cas9 is the efficient intracellular delivery of the editing machinery, which remains a well-recognized challenge, notably to deliver Cas9 in its native protein form. Herein, a phase-separating peptide with intracellular redox-triggered release properties is employed to encapsulate and deliver all three forms of CRISRP-Cas9 editing machinery, namely, pDNA, mRNA/sgRNA, and the ribonucleoprotein complex. These modalities are readily recruited within peptide coacervates during liquid-liquid phase separation by simple mixing and exhibit higher transfection and editing efficiency compared to highly optimized commercially available transfection reagents currently used for genome editing.
科研通智能强力驱动
Strongly Powered by AbleSci AI