Towards a better understanding of auxin response factors for improving cereal crops

The auxin response factor (ARF) is the key component of phytohormone auxin signal pathway and is crucial in regulating a plurality of functions throughout plant life cycle. Although ARFs’ structure and function have been well studied in Arabidopsis , such knowledge is far from being sufficient for cereal crops, especially wheat, rice and maize. This review is based on a comprehensive retrospection into the studies on ARFs in the three cereal crops, consisting of four parts: (1) characterization of the domains of 23, 25 and 33 ARF family members in wheat, rice and maize, respectively; (2) revision of nomenclatures for previously reported ARFs to the family numbers based on sequence alignment, and summary of ARFs’ functions including the regulation of agronomic traits and response to biotic/abiotic stresses; (3) highlight of general regulatory models for fundamental physiological and reproductive traits from miRNA- ARFs , IAA-ARF- LBD , IAA-ARF-Auxin response gene, and IAA-ARF- ERF (4) prospects to promising future ARF research for anticipated agronomic traits. Hopefully, the review will facilitate the understanding of ARF functions in the three cereal crops and promote its application in molecular breeding for achieving optimal plant architecture, higher yield, and wide adaptability.