Recent advances in glutathione fluorescent probes based on small organic molecules and their bioimaging

Glutathione (GSH), as one of the most important biological mercaptans, is involved in a variety of biological processes and is considered an important biomarker in early diagnosis, treatment and disease stage monitoring. Rapid and accurate detection of GSH in complex biological systems is of great significance for pathological analysis. Fluorescence imaging technology is widely used because of its advantages of high sensitivity, high resolution and non-destructiveness. In this paper, the latest research progress on GSH-responsive organic small molecule fluorescence probes in the last five years is summarized, and their response mechanisms are classified and discussed. In addition, the probe design strategy, sensing mechanism and biological application are discussed in this review. Finally, the challenges and future research directions of developing new GSH probes are presented.