Real-Time Monitoring of ONOO⁻ in Cerebral Ischemia-Reperfusion Injury Mouse Models Using a Hydrazine-Based NIR Fluorescent Probe

Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO-) in cerebral ischemia-reperfusion injury (CIRI) models are essential for understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO for detecting ONOO- rapidly and sensitively, with a low detection limit of 91 nM. Furthermore, TJO exhibits excellent fluorescence imaging capabilities, enabling detailed visualization of ONOO⁻ in CIRI mice model. This highlights its potential for real-time monitoring of ONOO⁻-related pathological conditions.