Clinical relevance of the plasma load of cytomegalovirus in patients infected with HIV-A survival analysis

To investigate whether asymptomatic cytomegalovirus (CMV) viraemia impact the course of human immunodeficiency virus (HIV) infection, this study evaluated the effect of CMV replication on progression of newly-diagnosed HIV infected individuals towards AIDS events and death. In a 3-year prospective study on co-infected patients, clinical, immunological, and virological tests were performed in a national reference hospital quarterly. CMV viraemia was quantified by RoboGene® HCMV DNA Quantification Kit (Analytik Jena, Germany), on ABI Prism® 7000 Sequence Detection System (Applied Biosystems, USA). One hundred and five patients were enrolled with a balanced sex distribution and a median age of 30.7 years. Median CD4+ cell count at enrollment was 164/mm3 and median HIV RNA 4.6 log10copies/ml. Detectable CMV viraemia was found in 25.7% of the patients. Kaplan–Meier analysis showed progression of HIV infection to be significantly increased in those with active CMV replication and/or low CD4+ cell count. Cox regression indicated the risk of developing new AIDS events was 2.6 times greater in patients with detectable CMV viraemia versus those without (CI95% 1–6.6; P = 0.04). Also in multivariate analysis, the overall risk of progression to AIDS events or death was 3-fold higher in those with detectable CMV viraemia (CI95% 1.3–6.7; P = 0.008) and 2.3-fold higher if CD4+ cell count was below 100/mm3 (CI95% 1–5.1; P = 0.04). In these young Romanian HIV-seropositives, active CMV replication increased morbidity, even when treated with combination antiretroviral therapy. Further studies are needed to evaluate if serial quantitative CMV-DNA levels might correlate with non-infectious inflammation-related risks in patients with HIV and active CMV infection. J. Med. Virol. 86:1821–1827, 2014. © 2014 Wiley Periodicals, Inc.