A simple and efficient transformation system for the edible mushroom Pleurotus eryngii

Although Pleurotus eryngii is a highly-valued mushroom, the tools for undertaking molecular biological research are lacking. We have now developed an improved, more efficient ATMT (Agrobacterium tumefaciens-mediated transformation) system for generating P. eryngii transformants. Successful generation of transformants required that nutrient-limited fungal mycelium be used as the receptor material. The transformants remained genetically stable and produced normal clamp connections in mating tests. The procedures developed here provide an effective and simple means for gene function studies and directed breeding in P. eryngii essential for realizing the full biotechnological potential of this mushroom.