动细胞
主轴检查点
细胞生物学
后期
微管
细胞周期检查点
染色体分离
生物
有丝分裂
主轴装置
化学
细胞分裂
细胞周期
细胞
遗传学
染色体
基因
作者
Zhejian Ji,Haixiao Gao,Hongtao Yu
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2015-06-12
卷期号:348 (6240): 1260-1264
被引量:170
标识
DOI:10.1126/science.aaa4029
摘要
The spindle checkpoint of the cell division cycle senses kinetochores that are not attached to microtubules and prevents precocious onset of anaphase, which can lead to aneuploidy. The nuclear division cycle 80 complex (Ndc80C) is a major microtubule receptor at the kinetochore. Ndc80C also mediates the kinetochore recruitment of checkpoint proteins. We found that the checkpoint protein kinase monopolar spindle 1 (Mps1) directly bound to Ndc80C through two independent interactions. Both interactions involved the microtubule-binding surfaces of Ndc80C and were directly inhibited in the presence of microtubules. Elimination of one such interaction in human cells caused checkpoint defects expected from a failure to detect unattached kinetochores. Competition between Mps1 and microtubules for Ndc80C binding thus constitutes a direct mechanism for the detection of unattached kinetochores.
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