Natural Immunostimulants from Chinese herbal Medicine and Thai Medicine for Brown Spotted Grouper

In our report,ethyl alcohol extraction method were performed to extract active ingredients from eight kinds of Chinese herb medicine(Epimedium herb(Epimedium sagittatum),Eucommia bark(Eucommia ulmoides),Radix astragali(Astragalus membranaceus),Lentinan(Lentinus edodes),Radix ginseng rubra(Panax ginseng),Five leaf Gynostemma herb(Gynostemma pentaphyllum),compound1(Shengmai Yu Ping Feng San),and compound2(Shi Quan Da Bu Tang)) and two kinds of Thai medicine(Turmeric(Curcuma longa) and Fahtalaijons(Andrographis paniculata));tetrazolium(NBT) reduction methods were performed to determine the production of superoxide anion and phagocytic activity of its white blood cell and analyze its immune effects for brown-spotted groupe(Epinephelus fuscoguttatus).The results indicated that with the extraction concentration of 10 g·L-1,Lentinan group showed the best immune effect;with the extraction concentration of 5 g·L-1,all experimental groups showed no significant immune effects;with the extraction concentration of 2.5 g·L-1 and 1.25 g·L-1,group Radix astragali,Lentinan,Radix ginseng rubra and compound1 obtained the most prominent immune effects;with the extraction concentration of 0.625 g·L-1,the immune effects of Radix astragali,Eucommia bark,and Turmeric group were optimal;the white blood cell phagocytic activity of Radix astragali,Lentinan,Radix ginseng rubra and compound1,were significantly higher than that of the control group.So,four kinds of natural immunostimulants for brown spotted grouper,Radix astragali,Lentinan,Radix ginseng rubra and Shengmai Yu Ping Feng San,respectively,were obtained in our report.These data were also very helpful for screening a new security immunostimulant for reared fish in future.