Determination of Ellagic Acid Content in Red Raspberry(Rubus corchorifolius Linn. f. ) Seed by HPLC

[Objective] The purpose was to determined content of ellagic acid extracted from red raspberry seed by HPLC.[Method] The chromatography conditions were as follows: C18 column of ODS HYPERSL(100.0 mm×4.6 mm at 5 μm),column temperature of 25 ℃,CH3OH-0.03% trifluoroacetic acid(volume ratio of 50∶50) as mobile phase and detection wavelength of 254 nm.[Result] The results showed that there was a good linearity between the peak area and the content of ellagic acid in the range of 0.322-8.050 μg(r=0.999 5).Precision test's RSD was 0.69%,showing instrument had good precision;Reproducibility test's RSD was 0.95%,showing determination method had good reproducibility;Average recovery of sample was 93.78%,RSD was 1.08%,showing determination method was accurate and reliable,could be used in subsequent analysis.And finally,the content of ellagic acid extracted from red raspberry seed was up to 994 mg/kg.[Conclusion] The content of ellagic acid in red raspberry seed is higher than that reported in the red raspberry fruit and its mechanism should be further studied.