Entecavir inhibits hepatitis B virus(HBV) replication in HBV replication mice

Objective To investigate whether HBV replication mouse model can be used for selection of anti-HBV medicine.Methods HBV replication-competent plasmid pHBV4.1 was transferred into male BALB/C mice by hydrodynamics-based in vivo transfection.The mice were matched by body weight,age and serum level of Hepatitis B e antigen(HBeAg) and were devided into experiment group and control group(4 mice in each group) 24 h after transfection and then treated orally with entecavir or normal saline at interval of twenty four hours.HBV mRNA and HBV DNA replication intermediates in liver were analyzed by Northern and Southern Blot Hybridization,respectively.The serum HBsAg and HBeAg were detected by enzyme linked immunosorbentassay(ELISA).Results The results of two indepent experiments showed that compared with the control animals,HBV DNA replicative intermediates dramatically decreased in the liver from the experiment mice.The levels of HBV mRNA from liver and theserum HBsAg and HBeAg,however,were not significantly different.Conclusion The inhibitory effect of entecavir on HBV was detected in the HBV replication mice,suggesting that the HBV replication mouse model can be used for selection of anti-HBV drugs.