Objective: To explore clinical application of repairing dural defects and intravascular embolization with lyophilized human dura mater. Methods: The lyophilized human dura mater was used to repair dural defects in brain trauma, brain tumor and spinal cord tumor. The target vessels were embolizated using microns and microfilaments made of lyophilized human dura mater. Results: The lyophilized human dura mater was used to repair dural defects in 229 patients. No complication including intracranial infection and foreign body reaction occurred after implantation. The target vessels was embolized using microns and microfilaments made of lyophilized human dura mater in 28 patients before operation. Conclusion: Lyophilized human dura mater is an ideal material to repair the dura mater defect or intravascular embolization.