Determination of Rutin and Quercetin in Tartary Buckwheat Extract

Objective:To determine the content of rutin and quercetin in the tartary buckwheat extract,so as to appraise its quality.Method: The chromatographic conditions were as follows: with the Welchrom-C18(4.6 mm × 150 mm,5 μm) as the chromatographic column,methanol-0.2% formic acid solution(50∶ 50) as the mobile phase,280 nm as the detection wavelength,30 ℃ as the column temperature,1.0 mL·min-1 as the flow rate.Result: The linear range for rutin was 1.56-100.00 mg·L-1 and the linear range for quercetin was 1.52-97.00 mg·L-1.The average recovery for rutin and quercetin was 98.49%(RSD 1.25%,n = 5) and 97.87%(RSD 1.69%,n = 5) respectively.Conclusion: Rutin and quercetin were 18.97% ± 1.43% and 5.03% ± 0.42% respectively determined by the method applied.