Diethylstilbestrol Occurrence in Eggs of Subcutaneously Injected Hens

Abstract Residues of diethylstilbestrol were shown to remain in eggs of laying hens for at least a week after the hens were injected with 25 mg of diethylstilbestrol in oil. Most of the drug occurred in the yolk. The diethylstilbestrol occurs free or in a loosely bound form readily cleaved by mild hydrochloric acid hydrolysis. Results of hydrolysis with beta-glucuronidase indicate that none of the drug occurs in the eggs as the glucuronide.