Fresh Datainong mango was treated with chitosan and 1-methylcyclopropene (1-MCP) during storage. The weight loss rate, hardness, soluble solid content, respiration rate, titratable acid content and chlorophyll content of mango during shelf-storage period were explored. Results indicated that the combinatorial treatment of chitosan and 1-MCP exhibited the highest respiration peak on the 10th day. Compared with CK, there was a delay for 4 days, and the content of titratable acids exhibited an increase of 4.4 folds. Chitosan and 1-MCP could also significantly reduce the softening degree of mango, decrease weight loss rate and chlorophyll content, attenuate the increase of soluble solids and maturity. Combinatorial treatments of chitosan and 1-MCP exhibited a better effect than chitosan or 1-MCP treatment alone, which will provide a significant improvement of mango quality during shelf-storage period.