机械敏感通道
超声波
神经刺激
体内
神经调节
小泡
生物医学工程
材料科学
生物物理学
纳米技术
聚焦超声
化学
离子通道
神经科学
刺激
医学
生物
膜
生物化学
放射科
生物技术
受体
作者
Xuandi Hou,Zhihai Qiu,Quanxiang Xian,Shashwati Kala,Jianing Jing,Kin Fung Wong,Jianguo Zhu,Jinghui Guo,Ting Zhu,Mingwei Yang,Lei Sun
标识
DOI:10.1002/advs.202101934
摘要
Ultrasound is a promising new modality for non-invasive neuromodulation. Applied transcranially, it can be focused down to the millimeter or centimeter range. The ability to improve the treatment's spatial resolution to a targeted brain region could help to improve its effectiveness, depending upon the application. The present paper details a neurostimulation scheme using gas-filled nanostructures, gas vesicles (GVs), as actuators for improving the efficacy and precision of ultrasound stimuli. Sonicated primary neurons display dose-dependent, repeatable Ca2+ responses, closely synced to stimuli, and increased nuclear expression of the activation marker c-Fos in the presence of GVs. GV-mediated ultrasound triggered rapid and reversible Ca2+ responses in vivo and could selectively evoke neuronal activation in a deep-seated brain region. Further investigation indicate that mechanosensitive ion channels are important mediators of this effect. GVs themselves and the treatment scheme are also found not to induce significant cytotoxicity, apoptosis, or membrane poration in treated cells. Altogether, this study demonstrates a simple and effective method to achieve enhanced and better-targeted neurostimulation with non-invasive low-intensity ultrasound.
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