Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology

Response surface methodology(RSM) was used to optimize the cellulase-assisted extraction process of total flavonoids(TF) from pigeon pea leaves.The amount of enzyme,enzymatic hydrolysis temperature,time and pH were selected as influencing factors and TF yield as the response value based on single factor trials.The experiment mathematical model was established according to Box-Behnken central composite experiment design and was analyzed by RSM.The results showed the optimal extraction conditions were as follows:enzyme amount 8.65 mg,extraction temperature 33.88℃,extraction time 2.02 h and extraction pH 5.02.The predicted value of TF yield was 4.86% under the optimum conditions,and the experimental value was 4.88%.The optimal extraction conditions were stable and reliable,and would provide experimental basis for the future development of TF in pigeon pea leaves.