合胞滋养细胞
细胞滋养层
氧气张力
细胞生物学
滋养层
生物
合胞体
胎盘
人胎盘催乳素
细胞分化
分子生物学
细胞
内科学
化学
生物化学
氧气
胎儿
医学
怀孕
遗传学
有机化学
基因
作者
Jared C. Robins,Angela B. Heizer,Atira Hardiman,Michael Hubert,Stuart Handwerger
出处
期刊:Placenta
[Elsevier]
日期:2007-11-01
卷期号:28 (11-12): 1141-1146
被引量:31
标识
DOI:10.1016/j.placenta.2007.05.006
摘要
During placental development, human cytotrophoblast cells can differentiate to either villous syncytiotrophoblast cells or invasive extravillous trophoblast cells. We hypothesize that oxygen tension plays a critical role in determining the pathway of cytotrophoblast differentiation. A highly purified preparation of cytotrophoblast cells from human third trimester placenta was cultured for 5 days in either 20% or 1% oxygen tension. The cells incubated at 20% oxygen formed a syncytium as determined by immunohistochemistry using an anti-desmosomal protein antibody that identifies cell membranes. In addition, the mRNA was markedly induced for syncytin, a glycoprotein shown to be essential for syncytiotrophoblast formation, and for human placental lactogen (hPL), which is a specific marker for syncytiotrophoblast cells. In contrast, the cell incubated at 1% oxygen tension did not fuse by morphologic analysis and did not express syncytin or hPL mRNA. However, these cells expressed abundant amounts of HLA-G, a specific marker for extravillous trophoblast cells, which was not seen in cells incubated at 20% oxygen tension. These results suggest that low oxygen tension directs differentiation along the extravillous trophoblast cell pathway while greater oxygen tension directs differentiation along the villous trophoblast cell pathway.
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