Identification and quantitative determination of eudesmane-type acids from the essential oil ofDittrichia viscosa sp.viscosa using13C-NMR spectroscopy

A procedure that allows the identification and quantitative determination of eudesmane-type acids in the acidic part of the essential oil of Dittrichia viscosa sp. viscosa is described. The method involves the computer-aided analysis of the 13C-NMR spectrum of the mixture without the requirement of previous separation or derivatisation. The quantitative procedure was verified with costic acid standard and applied to three other acids which possess the same eudesmane framework. Copyright © 2005 John Wiley & Sons, Ltd.