毛细管电色谱
毛细管电泳
特里斯
化学
色谱法
共价键
羟甲基
适体
毛细管作用
连接器
相(物质)
电泳
寡核苷酸
理论版
电色谱法
分析化学(期刊)
DNA
材料科学
立体化学
有机化学
生物化学
复合材料
操作系统
生物
遗传学
计算机科学
作者
Melanie A. Rehder,Linda B. McGown
标识
DOI:10.1002/1522-2683(200109)22:17<3759::aid-elps3759>3.0.co;2-c
摘要
DNA aptamers that form a G-quartet conformation were covalently attached to a capillary surface for open-tubular capillary electrochromatographic separation of bovine β-lactoglobulin variants A and B, which vary by 2 of their 162 amino acid residues. Separation was achieved using a 4-plane, G-quartet aptamer stationary phase with tris(hydroxymethyl)aminomethane (Tris) or phosphate buffer as the mobile phase. In control experiments, separation did not occur using either an oligonucleotide of similar base composition but which does not form a G-quartet structure, or using capillary zone electrophoresis on a bare capillary under similar experimental conditions. Separation was achieved using a capillary coated only with the covalent linker molecule. In phosphate buffer, the separations were similar for aptamer-coated and linker-only stationary phases, while in Tris buffer, retention times were almost doubled for the linker-only capillary. When Tris buffer is the mobile phase, there appears to be weaker interactions between the proteins and the stationary phase that may result in a gentler, less denaturing separation than is commonly achieved using hydrocarbon-based stationary phases.
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