检出限
分子印迹聚合物
化学
抗体
组合化学
色谱法
生物化学
选择性
生物
催化作用
免疫学
作者
Zhaorui Song,Xin-Hui Zhai,Chenrui Jiang,Rong Chen,Sijing Ye,Jinzhe Tong,Pierre Dramou,Hua He
标识
DOI:10.1016/j.bios.2022.114037
摘要
A novel bionic enzyme-linked immunosorbent assay (BELISA) based on double-antibody sandwich method is firstly designed for the detection of carbamazepine (CBZ) in human serum samples. In this BELISA system, cucurbit[7]uril (CB[7]) is employed as an artificial capture antibody (cAb), and molecularly imprinted polymers (MIPs) is used as an artificial detection antibody (dAb). Nanozymes (PdNPs) as signal generators are integrated with MIPs. This couple of bionic antibodies exhibits not only the excellent physical and chemical stability, but also the superior molecular recognition ability. Based on two bionic antibodies that can selectively recognize different sites of CBZ molecule, a new BELISA method has been constructed for the first time. The proposed BELISA method displays a good linear relationship ranging from 2 to 20 μg mL-1. The detection limit is 0.37 μg mL-1, which can well meet clinical testing demand. It provides a more stable and economical method for clinical therapeutic drug monitoring (TDM).
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