CFU/fish for S36 (Songkhla). Histopathology revealed encephalitis, myocarditis and renal tubular necrosis. All isolates were highly susceptible to tested antimicrobials. These findings highlight S. iniae as a new pathogen in spotted scat aquaculture in Thailand and emphasise the need for integrated management focused on water quality, parasite control and husbandry practices to mitigate streptococcosis risk.