Placental Vascular Defects and Embryonic Lethality Triggered by TFPIα Deficiency in Factor V Leiden Mice

BACKGROUND: TFPI (tissue factor pathway inhibitor) inhibits the initiation of blood coagulation. TFPIα (TFPI alpha isoform), the only alternatively spliced TFPI isoform in platelets, is abundant in placenta and uniquely inhibits prothrombinase (FXa [activated factor X]-FVa). This inhibitory activity is reduced when prothrombinase is assembled with FVL (factor V Leiden). METHODS: Effects of TFPIα ( Tfpi Δα ) and platelet ( Tfpi fl ; Pf4 -Cre + ) specific knockout alleles were characterized in FVL (F5 L ) mice to examine the physiological effects of the TFPIα-FV interaction. RESULTS: Genotype frequencies were assessed and revealed that Tfpi +/Δα F5 L/L mice survive to adulthood. However, Tfpi Δα homozygosity with even a single F5 L allele resulted in embryonic lethality during mid-gestation development regardless of maternal FVL status. In contrast, F5 L/L Tfpi fl/fl Pf4 -Cre + mice were at expected frequencies at weaning, indicating that platelet TFPIα loss alone did not cause mid-gestation lethality in Tfpi Δα/Δα F5 L mice. Histological analyses showed no fibrin deposition in embryonic or extraembryonic tissues but revealed placental vasculature defects in Tfpi Δα/Δα F5 L genotypes. Treatment with the direct thrombin inhibitor dabigatran partially rescued the lethality and corrected placental defects, implicating excessive thrombin generation as a factor in Tfpi Δα/Δα F5 L demise. CONCLUSIONS: These findings suggest that TFPIα and its inhibition of prothrombinase play an important role in placental angiogenesis and embryonic survival.