生物分析
动力学
材料科学
环介导等温扩增
纳米技术
连锁反应
黄曲霉毒素
DNA
脱氧核酶
化学
光化学
生物化学
物理
食品科学
量子力学
作者
Xuesong Li,Jie Cheng,Kun Zeng,Shulin Wei,Jiaxuan Xiao,Yao Lu,Fuguo Zhu,Zhanhui Wang,Kun Wang,Xiangyang Wu,Zhen Zhang
标识
DOI:10.1021/acsami.3c05506
摘要
Traditional hybridization chain reaction (HCR) as a popular isothermal amplification technique shows some inevitable disadvantages in bioanalysis due to its relatively slow kinetics, which could be markedly promoted when the HCR initiator occurs under tension. Herein, a poly DNA tetrahedrons (pTDNs)-mediated HCR was successfully constructed to make its initiator in a stretched state by long-range electrostatic forces owing to the superimposed electrostatic interactions derived from the synthesized pTDNs, and it was hypothesized that it could remarkably enhance HCR performance, which was testified by theoretical simulations and experimental studies. Consequently, pTDNs-mediated HCR was applied to develop a novel immunoassay for rapid and sensitive detection of aflatoxin B1 as a proof-of-concept, and its signal amplification was attributed to the increased G4 DNAzyme that loaded on the second antibody. Our work paves a promising way using simple DNA frameworks alone to heighten HCR kinetics for reaction speed improvement and signal amplification in bioanalysis.
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