化学
乙酰胆碱酯酶
铂纳米粒子
组合化学
金属有机骨架
催化作用
过氧化物酶
纳米颗粒
铂金
纳米技术
无机化学
酶
有机化学
材料科学
吸附
作者
Yinhui Yi,Xun Zhou,Diyan Liao,Jingguo Hou,Hongde Liu,Gangbing Zhu
出处
期刊:Inorganic Chemistry
[American Chemical Society]
日期:2023-08-16
卷期号:62 (34): 13929-13936
被引量:4
标识
DOI:10.1021/acs.inorgchem.3c01844
摘要
The sensitive detection of acetylcholinesterase (AChE) and organophosphorus pesticides (OPs) is very important for the protection of human health. Herein, a hybrid material, Pt NPs/Fe-MOF, consisting of a metal–organic framework (MIL-88B-NH2, Fe-MOF) decorated with platinum nanoparticles (Pt NPs), was prepared first and exhibited remarkably improved and excellent peroxidase-mimicking activity compared to the Fe-MOF material resulting from the synergistic catalysis effect between Fe-MOF and Pt NPs, which can effectively catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to generate a blue product (oxidized TMB, oxTMB). Interestingly, in the presence of AChE and acetylcholinesterase, the peroxidase-mimicking activity from Pt NPs/Fe-MOF was inhibited obviously, and thus, a colorimetric sensing platform for AChE can be constructed; more importantly, after the addition of OPs, this nanozyme activity can be recovered, inducing the further successful construction of a sensitive colorimetric sensing platform for OPs. The related sensing mechanism and condition optimization were studied, and the as-prepared Pt NPs/Fe-MOF nanozyme-based colorimetric method for AChE and OP detection displayed superior analytical performances with wide linearities and low detection limits. Furthermore, the designed method offers satisfactory real application ability. We expect the as-proposed Pt NPs/Fe-MOF nanozyme-based colorimetric sensing platform for AChE and OPs via the enzyme cascade reaction to show great potential application.
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