蛋白激酶B
癌症研究
PI3K/AKT/mTOR通路
马尔克斯
上皮-间质转换
癌变
转移
信号转导
医学
癌症
生物
细胞生物学
蛋白激酶C
内科学
作者
Lixia Tang,Peng-Cheng Ye,Yu Lin,Yang Luo,Wanlong Tan,Wanping Xiang,Zilin Liu,Lei Tan,Jiangwei Xiao
出处
期刊:World Journal of Gastrointestinal Oncology
[Baishideng Publishing Group Co (World Journal of Gastrointestinal Oncology)]
日期:2023-08-15
卷期号:15 (8): 1366-1383
标识
DOI:10.4251/wjgo.v15.i8.1366
摘要
Long non-coding RNAs (lncRNAs) with differential expression characteristics have been found to be closely related to the tumorigenesis and development of gastric cancer (GC), but their specific mechanisms and roles still need to be further elucidated.To investigate the expression of LINC01268 in GC and its mechanism of affecting GC progression.Real-time quantitative polymerase chain reaction was used to detect the expression of LINC01268 in GC tissues, cell lines and plasma. The Kaplan-Meier method was used to evaluate the value of LINC01268 in the prognostication of GC patients. An receiver operating characteristic curve was constructed to evaluate the value of LINC01268 in the diagnosis of GC. Transwell migration and invasion assays and wound healing assays were used to confirm the effect of LINC01268 on the invasion and migration of GC cells. The regulatory relationship between LINC01268 and myristoylated alanine rich protein kinase C substrate (MARCKS), the PI3K/Akt signaling pathway, and the epithelial-mesenchymal transition (EMT) process in GC was demonstrated by western blot analysis.The expression of LINC01268 was increased in GC tissues and cell lines. The expression level of LINC01268 was significantly correlated with lymph node metastasis, TNM stage, and tumor differentiation in patients with GC. Over-expression of LINC01268 indicated a poor prognosis for patients with GC, and it had a certain auxiliary diagnostic value for GC. In vitro functional experiments proved that the abnormal expression of LINC01268 further activated the PI3K/Akt signaling pathway and promoted EMT by targeting and regulating MARCKS and ultimately promoted the invasion and metastasis of GC.This study elucidates that LINC01268 in GC may be an oncogene that further activates the PI3K/Akt signaling pathway and EMT by targeting and regulating MARCKS, and ultimately promotes the invasion and metastasis of GC. LINC01268 may be a potential effective target for the treatment of GC.
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