微泡
脐静脉
脐带
子痫前期
胎盘
氧化应激
男科
滋养层
血管生成
内皮功能障碍
医学
内分泌学
细胞生物学
内科学
免疫学
生物
体外
胎儿
怀孕
小RNA
生物化学
基因
遗传学
作者
Mengqi Gu,Fengyuan Zhang,Xiaotong Jiang,Pengzheng Chen,Shuting Wan,Qingfeng Li,Yuan Lu,Qian Zhou,Yanyun Wang,Lei Li
标识
DOI:10.3389/fcvm.2022.1061340
摘要
Early onset preeclampsia (EOSP, PE) is characterized by hypertension, proteinuria, and endothelial dysfunction. Oxidative stress-induced trophoblast dysfunction is a major pathology in PE. Placental exosomes are extracellular vesicles that are involved in "mother-placenta-foetal communication" and can regulate the biological functions of endothelial cells. Our study was designed to evaluate placental exosomes effects on endothelial cells.Umbilical cord blood from normal pregnant women and patients with PE were collected. A hypoxia/reoxygenation (H/R) model in human first trimester extravillous trophoblast cell (HTR8/SVneo) line to simulate the PE model of oxidative stress in vitro. Then, placental exosomes (i.e., NO-exo, H/R-exo, N-exo, and PE-exo) were extracted and identified. Finally, the effects of placental exosomes on the biological functions of human umbilical vein endothelial cells (HUVECs) were further evaluated by performing a series of experiments.Placental exosomes had a double-membrane cup structure with diameters of 30-150 nm, and there was no obvious difference in placental exosomes. Compared with NO-exo and N-exo, H/R-exo and PE-exo inhibited HUVECs proliferation, tube formation and migration, increased permeability and apoptosis in vitro.We hypothesize that H/R-exo and PE-exo impair vessel development by disrupted biological functions in endothelial cells, which may result in vascular disorders in offspring.
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