Improving the effect of Topical and Systemic administration of Probiotic Lactobacillus casei Shirota on Interleukin-10 Expression in Traumatic Ulcer Healing in Wistar Rats (Rattus norvegicus)

Background: The use of antibiotics, steroids, and anti-inflammatories as a therapy for traumatic ulcers can cause resistance and side effects. Furthermore, its application is limited to a few general conditions in patients, necessitating the use of alternative treatments based on natural ingredients and probiotics. Administration of the probiotic Lactobacillus casei Shirota can affect the increase in IL-10 expression in the process of wound healing. Purpouse: The goal of this study is to show that topical and systemic administration of the probiotic Lactobacillus casei Shirota improves the healing of traumatic ulcers in Wistar rats. Methods: A laboratory experimental study of 36 healthy male Wistar rats, 2-3 months old, with a 175-250gram body weight, divided into 6 groups. In all groups, traumatic ulcers were made in the labial region of the inferior incisive fornix using a heated round burner tip. The control group was given Aquadest, and the topical and systemic treatment groups were given probiotics, at a dose of 10.9 x 107cells/kg body weight every day for 4 days and 8 days for traumatic ulcers. The amount of IL-10 expression was observed by IHC examination followed by a one-way ANOVA test. Results: There was a significant difference between the 14-day topical treatment group and the 4-day systemic treatment group (p = 0.000) and between the 8-day topical treatment group and the 8-day systemic treatment group (p = 0.005). In the 4 and 8-day topical treatment groups, there was an increase in the mean amount of IL-10 with a significant difference (p = 0.000). Conclusion: There was an increase in IL-10 expression after administration of the probiotic Lactobacillus casei Shirota in the topical treatment group compared to the control and systemic treatment groups in the healing of traumatic ulcers in Wistar rats (Rattus norvegicus).