去卵巢大鼠
间充质干细胞
衰老
骨髓
干细胞
自噬
内分泌学
内科学
油红O
男科
雌激素
生物
化学
医学
脂肪生成
细胞凋亡
细胞生物学
生物化学
作者
Liang Bing,Xiongbin Chen,Min Lü,Lingling Zhang,Xiaohong R. Yang,Lei Shi,Yanju Gong,Gong Yang,Hanlin Xu,Xiao Wu,Zhong Jin,Yanru Wang,L. Liu,Xiaohong Yi,Lushuang Xie,Hua Zhong,Chongyang Shen,Y Wang,Yang Liu
标识
DOI:10.1080/13880209.2023.2291675
摘要
Materials and methods Forty-eight female Sprague-Dawley rats were divided into four groups: sham-operated (SHAM), ovariectomized (OVX), LWDH high dose (LWDH-H, 1.6 g/kg/d) and LWDH low dose (LWDH-L, 0.8 g/kg/d); the doses were administered after ovariectomy via gavage for eight weeks. After eight weeks, the bone microarchitecture was evaluated. The effect of LWDH on the differentiation of bone marrow mesenchymal stem cells (BMSCs) was assessed via osteogenesis- and lipogenesis-induced BMSC differentiation. The senescence-related biological indices were also detected using senescence staining, cell cycle analysis, quantitative real-time polymerase chain reaction and western blotting. Finally, the expression levels of autophagy-related proteins and Yes-associated protein (YAP) were evaluated.
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