Improvement of Cross-linked Alginate Hydrogel Microparticles Loaded with Galangin-β-Cyclodextrin Complex by Response Surface Methodology

Abstract Galangin is a phenolic compound with antioxidant and anti-tyrosinase activity, which makes it highly useful in cosmetics and medicine. However, the poor solubility of galangin in water limits its usefulness in these areas. This study it is aimed to increase the solubility of galangin in water by encapsulation method. Therefore, in this study, ethanolic and methanolic extracts were obtained from Alpinia officinarum Hance, and the phenolic compound profile and content of the extracts were determined by HPLC-DAD. Galangin was purified and fractionated from Alpinia officinarum Hance extracts by column chromatography. Galangin was encapsulated with β-cyclodextrin, and galangin-β-cyclodextrin loaded alginate hydrogel microparticles were developed. The central composite design-response surface methodology was used to develop galangin-β-cyclodextrin loaded alginate hydrogel microparticles under optimum conditions with maximum galangin release. The encapsulation efficiency and release of galangin in galangin-β-cyclodextrin loaded alginate hydrogel microparticles developed under optimum conditions were characterized by HPLC-DAD, surface morphology by SEM, and structural properties by FTIR.