Designing Effective Antimicrobial Agents: Structural Insights into the Antibiofilm Activity of Ionic Liquids

Research concerning biofilm control is critical due to the pervasive and resilient nature of biofilms, which pose significant challenges across the industrial, environmental, and healthcare sectors. Traditional antimicrobial treatments are often ineffective against these robust structures. Here, we explore the antimicrobial properties of ionic liquids (ILs) and their efficacy in biofilm disruption. By examining the structural variations of ILs, we highlight the key role of hydrophobicity, noting that longer alkyl side chains in IL cations enhance biofilm disruption and bacterial death. However, upon reaching a certain optimal chain length─usually C12 to C14─the antimicrobial activity of ILs starts to decrease. Furthermore, the symmetry and size of anions significantly impact biofilm elimination. This Perspective addresses a critical gap in biofilm research, revealing the structure–activity relationships of ILs and providing a foundation for designing more effective biofilm-disrupting agents.