克拉斯
粒体自噬
癌症研究
胰腺癌
胰腺上皮内瘤变
线粒体
癌症
生物
基因敲除
自噬
细胞生物学
癌变
结直肠癌
细胞凋亡
生物化学
遗传学
作者
Timothy J. Humpton,Brinda Alagesan,Gina M. DeNicola,Dan Lü,Georgi N. Yordanov,Carl S. Leonhardt,Melissa A. Yao,Maya N. Zaatari,Young-Kyu Park,Jeremy N. Skepper,Kay F. Macleod,Pedro A. Pérez–Mancera,Michael P. Murphy,Gérard I. Evan,Karen H. Vousden,David A. Tuveson
出处
期刊:Cancer Discovery
[American Association for Cancer Research]
日期:2019-09-01
卷期号:9 (9): 1268-1287
被引量:122
标识
DOI:10.1158/2159-8290.cd-18-1409
摘要
Abstract Activating KRAS mutations are found in nearly all cases of pancreatic ductal adenocarcinoma (PDAC), yet effective clinical targeting of oncogenic KRAS remains elusive. Understanding of KRAS-dependent PDAC-promoting pathways could lead to the identification of vulnerabilities and the development of new treatments. We show that oncogenic KRAS induces BNIP3L/NIX expression and a selective mitophagy program that restricts glucose flux to the mitochondria and enhances redox capacity. Loss of Nix restores functional mitochondria to cells, increasing demands for NADPH reducing power and decreasing proliferation in glucose-limited conditions. Nix deletion markedly delays progression of pancreatic cancer and improves survival in a murine (KPC) model of PDAC. Although conditional Nix ablation in vivo initially results in the accumulation of mitochondria, mitochondrial content eventually normalizes via increased mitochondrial clearance programs, and pancreatic intraepithelial neoplasia (PanIN) lesions progress to PDAC. We identify the KRAS–NIX mitophagy program as a novel driver of glycolysis, redox robustness, and disease progression in PDAC. Significance: NIX-mediated mitophagy is a new oncogenic KRAS effector pathway that suppresses functional mitochondrial content to stimulate cell proliferation and augment redox homeostasis. This pathway promotes the progression of PanIN to PDAC and represents a new dependency in pancreatic cancer. This article is highlighted in the In This Issue feature, p. 1143
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