An in situ hydrogel based on carboxymethyl chitosan and sodium alginate dialdehyde for corneal wound healing after alkali burn

材料科学 壳聚糖 角膜 移植 伤口愈合 体内 生物医学工程 细胞毒性 组织工程 原位 体外 化学 外科 生物化学 眼科 有机化学 医学 生物 生物技术
作者
Wu Xu,Kaibin Liu,Tong Li,Wenhua Zhang,Yanhan Dong,Jiayi Lv,Wenli Wang,Jialin Sun,Mengjie Li,Meng Wang,Zihong Zhao,Ye Liang
出处
期刊:Journal of Biomedical Materials Research Part A [Wiley]
卷期号:107 (4): 742-754 被引量:61
标识
DOI:10.1002/jbm.a.36589
摘要

Abstract There is currently no optimal scaffold for the transplantation of limbal stem cells (LSCs) to induce corneal reconstruction after corneal alkali burns. This study attempts to fabricate a novel in situ Alginate‐Chitosan hydrogel (ACH) for LSCs transplantation. Sodium alginate dialdehyde (SAD), a biological crosslinker, was prepared by periodate‐mediated sodium alginate oxidization. Carboxymethyl chitosan was rapidly crosslinked with SAD via Schiff's base formation between the available aldehyde and amino groups. The ACH is rapidly formed on the wound surface by self‐crosslinking without adding any chemical crosslinking component. Gelation time, transmittance, microscopic structure, equilibrium swelling, cytotoxicity, histocompatibility and degradability of the hydrogel were all examined. Rabbit primary LSCs were encapsulated in the hydrogel and transplanted to alkali burn wounds in vivo . Cornea reconstruction was evaluated by visual observation, slit lamp, histological analysis, and immunofluorescence staining. Results showed that the in situ hydrogel was highly transparent, gelated quickly, biocompatible, and had low cytotoxicity. LSCs cultured in vitro expressed the stem marker p63 but lacked the differentiated epithelial markers cytokeratin 3 and 12. Furthermore, the hydrogel encapsulating LSCs could be formed quickly on the alkali burn wound of the cornea and was shown to significantly improve epithelial reconstruction. Taken together, treatment with this novel in situ hydrogel‐mediated LSC transplantation system may serve as a rapid and effective method for corneal wound healing. © 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 742–754, 2019.
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