A sensitive and selective fluorescent probe for the detection of endogenous peroxynitrite (ONOO−) in living cells

Peroxynitrite (ONOO-) is one of reactive oxygen species, and plays a vital role in numeorus physiological and pathological processes. Given that the ONOO- level is closely related with various serious diseases, the in situ and real time detection of endogenous ONOO- is highly important for the in-depth study of its roles in living systems. Herein, we present a new fluorescent probe (RHPN) for the real-time detection of intracellular ONOO-. The probe RHPN consists of a rhodamine analogue and an arylhydrazide group as a response site for ONOO-. In response to ONOO-, the probe RHPN converts to an open-ring form and generates strong fluorescence. Moreover, the probe RHPN was successfully used for the imaging of the endogenous and exogenous ONOO- level changes in living cells.