运行x2
细胞生物学
间充质干细胞
生物
利基
平衡
信号转导
转录因子
生态学
遗传学
基因
作者
Shuo Chen,Junjun Jing,Yuan Yuan,Jifan Feng,Xia Han,Quan Wen,Thach‐Vu Ho,Chelsea Lee,Yang Chai
出处
期刊:Cell Reports
[Cell Press]
日期:2020-08-01
卷期号:32 (6): 108007-108007
被引量:50
标识
DOI:10.1016/j.celrep.2020.108007
摘要
Stem cell niches provide a microenvironment to support the self-renewal and multi-lineage differentiation of stem cells. Cell-cell interactions within the niche are essential for maintaining tissue homeostasis. However, the niche cells supporting mesenchymal stem cells (MSCs) are largely unknown. Using single-cell RNA sequencing, we show heterogeneity among Gli1+ MSCs and identify a subpopulation of Runx2+/Gli1+ cells in the adult mouse incisor. These Runx2+/Gli1+ cells are strategically located between MSCs and transit-amplifying cells (TACs). They are not stem cells but help to maintain the MSC niche via IGF signaling to regulate TAC proliferation, differentiation, and incisor growth rate. ATAC-seq and chromatin immunoprecipitation reveal that Runx2 directly binds to Igfbp3 in niche cells. This Runx2-mediated IGF signaling is crucial for regulating the MSC niche and maintaining tissue homeostasis to support continuous growth of the adult mouse incisor, providing a model for analysis of the molecular regulation of the MSC niche.
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