Live‐Cell Imaging: Time‐Gated FRET Nanoprobes for Autofluorescence‐Free Long‐Term In Vivo Imaging of Developing Zebrafish (Adv. Mater. 39/2020)

In article number 2003912, Marcelina Cardoso Dos Santos, Niko Hildebrandt, and co-workers develop terbium-to-quantum-dot fluorescence resonance energy transfer (FRET) nanohybrids for bright and autofluorescence-free multiplexed imaging in live cells and long-term imaging in developing zebrafish embryos over seven days, upon one-cell stage microinjection. The work provides a strategy for advancing in vivo imaging applications beyond the capabilities of fluorescent proteins.