甘氨酸
化学
过氧化物酶
线性范围
Zeta电位
核化学
检出限
无机化学
纳米技术
色谱法
酶
氨基酸
有机化学
纳米颗粒
生物化学
材料科学
作者
Wen‐Fei Dong,Liaoyuan Yang,Yuming Huang
出处
期刊:Talanta
[Elsevier]
日期:2017-05-01
卷期号:167: 359-366
被引量:64
标识
DOI:10.1016/j.talanta.2017.02.039
摘要
A facile and rapid post-synthetic strategy was proposed to prepare a glycine functionalized MIL-53(Fe), namely glycine-MIL-53(Fe), by a simple mixing of water dispersible MIL-53(Fe) and glycine. The FT-IR, SEM, XRD and zeta potential were used to characterize the glycine-MIL-53(Fe). The result showed that glycine post-synthetic modification of MIL-53(Fe) did not change in the morphology and crystal structure of MIL-53(Fe). Interestingly, compared with MIL-53(Fe), the glycine-MIL-53(Fe) exhibits an enhanced peroxidase-like activity, which could catalyze the oxidation of TMB by H2O2 to produce an intensive color reaction. Kinetic analysis indicated that the Km of glycine-MIL-53(Fe) for TMB was one-tenth of that of MIL-53(Fe). The glycine-MIL-53(Fe) as peroxidase mimetic displays better stability under alkaline or acidic conditions than MIL-53(Fe). The good performance of glycine-MIL-53(Fe) over MIL-53(Fe) may be attributed to the increase of affinity between TMB and the glycine-MIL-53(Fe). With these characteristics, a simple and sensitive method was developed for the detection of H2O2 and glucose. The linear detection range for H2O2 is 0.10-10μM with a detection limit of 49nM, and glucose could be linearly detected in the range from 0.25 to 10μM with a detection limit of 0.13μM. The proposed method was successfully used for glucose detection in human serum samples.
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