脂滴
溶剂变色
细胞内
生物物理学
赫拉
荧光
化学
斑马鱼
转导(生物物理学)
染色
尼罗河红
生物化学
分子
体外
有机化学
生物
物理
遗传学
量子力学
基因
作者
Maohua Wang,Wei-Long Cui,Yun-Hao Yang,Jian-Yong Wang
出处
期刊:Biosensors
[MDPI AG]
日期:2022-10-09
卷期号:12 (10): 851-851
被引量:5
摘要
Lipid droplets (LDs) are simple intracellular storage sites for neutral lipids and exhibit important impact on many physiological processes. For example, the changes in the polar microenvironment inside LDs could affect physiological processes, such as lipid metabolism and storage, protein degradation, signal transduction, and enzyme catalysis. Herein, a new fluorescent chemo-sensor (Couoxo-LD) was formulated by our molecular design strategy. The probe could be applied to effectively label intracellular lipid droplets. Intriguingly, Couoxo-LD demonstrated positive sensitivity to both polarity and viscosity, which might be attributed to its D-π-A structure and the twisted rotational behavior of the carbon-carbon double bond (TICT). Additionally, Couoxo-LD was successfully implemented in cellular imaging due to its excellent selectivity, pH stability, and low biotoxicity. In HeLa cells, the co-localization curve between Couoxo-LD and commercial lipid droplet dyes overlapped at 0.93. The results indicated that the probe could selectively sense LDs in HeLa cells. Meanwhile, Couoxo-LD can be applied for in vivo imaging of zebrafish.
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