The anti-aging mechanisms of Catalpol by elevating mitophagy

• Enhancement of Cell Viability : Treatment with Catalpol (CAT) significantly increases the viability of yeast cells, suggesting its potential role as an anti-aging agent by promoting cell health and function. • Identification of HSP82 as a Key Target : RNA-Seq and bioinformatics analyses identified HSP82 as a critical target of CAT, linking it to the regulation of mitophagy and mitochondrial activity in the context of aging. • HSP82 -Dependent Mitophagy : The study demonstrated that CAT enhances mitochondrial functions and extends the lifespan of yeast cells via HSP82 -dependent mitophagy, highlighting its importance in cellular maintenance. • Mechanistic Insights into Anti-Aging Effects : The research reveals the molecular mechanisms through which CAT exerts anti-aging effects, particularly by modulating mitophagy pathways to improve cell longevity. • Potential for Treating Age-Related Diseases : Findings indicate that CAT could serve as a promising therapeutic candidate for addressing age-related diseases by enhancing mitophagy and promoting mitochondrial health. Autophagy is an important mechanism for maintaining cell homeostasis, and its decline in function is widely regarded as a key factor in aging. Catalpol (CAT), an active compound derived from the traditional Chinese herb Rehmannia glutinosa , has shown potential to treat various age-related diseases through the regulation of autophagy. However, how CAT exerts anti-aging effects by regulating autophagy remains unclear. Therefore, this study sought to explore the anti-aging potential and molecular mechanisms, particularly the role of CAT in autophagy and mitochondrial function in yeast cells. The PI-CLS measurement method was employed to demonstrate enhanced yeast cells viability upon CAT treatment. RNA-Seq combined with bioinformatics analyses identified HSP82 as a key target of CAT. HSP82 knockout and HSP82 overexpression strains were employed to demonstrate that CAT enhances mitochondrial functions and extends cells lifespan by inducing HSP82 -dependent mitophagy. We concluded that CAT plays an anti-aging role by regulating HSP82 -dependent mitophagy and mitochondrial functions in yeast cells.