Complete genome sequence of a novel endornavirus from Rhizoctonia solani AG-1 IA isolate MY-JK-1

In this paper, we report a new fungal virus, tentatively named “Rhizoctonia solani endornavirus 15” (RsEV 15). The virus is a new type of fungal virus isolated from Rhizoctonia solaniAG-1 IA strain MY-JK-1. RsEV 15 is a positive single-stranded RNA (+ssRNA) composed of 18,934 nucleotides and contains two ORFs. ORF 1 encodes a large polypeptide of 5070 amino acids with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF 2 encodes an unknown functional protein composed of 1063 amino acids. The results of BLASTp search showed that the polypeptide sequence encoded by ORF 1 had the highest similarity with other sequences, and the homology with Rhizoctonia solani endornavirus 8 (RsEV 8) was 43.54 %. Multiple sequence alignment and phylogenetic analysis based on RdRp and Hel sequences showed that RsEV 15 may be a new member of Endornavirus.