rPDAs doped antibacterial MOF-hydrogel: bio-inspired synergistic whole-process wound healing

Wound healing as a fundamental health problem involves complex and dynamic biological processes. Multifunctional dressings that meet the needs of different wound stages can exert synergistic effects throughout the whole-process healing. In this work, inspired by the behavior of spider hunting, reduced polydopamine nanoparticles (rPDA) doped copper-based metal-organic frameworks (Cu-MOF)-hydrogel (GEL-MOF-rPDA) was prepared with anti-infection and promotion of whole-process wound healing. Schiff base cross-linking of dodecyl chitosan -oxidized sodium alginate was used as a biocompatible hydrogel substrate for hemostasis during wound healing and for steric fixation of bacteria during synergistic antibacterial processes. The dodecyl tail can be inserted and fixed on the lipid bilayer of the cell membrane while achieving bacterial capture, blood cell fixation, and skin cell fixation. Cu-MOF and rPDAs played in the hydrogel destroyed the outer membrane of bacteria and release 1O2 and Cu2+. The composite hydrogel had shown an excellent synergistic antibacterial effect with a 99.9% antibacterial rate. Furthermore, the mechanism of promoting whole-process wound healing was analyzed using an infected rat full-thickness skin defect model. The wound closure rate of the composite hydrogel treated wound was found as 92% at ten days. The bio-inspired synergistic antibacterial hydrogel solves the limitation of single-functional hydrogel in promoting whole-process wound healing through initial hemostasis, mid-term anti-infection and anti-inflammatory, late-stage anti-oxidation and promoting angiogenesis and granulation tissue formation. This biomimetic hydrogel paves the way for the design of multifunctional hydrogels and has good potential value in clinical applications.